H2-K1 Knockout cell line (MC38)

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H2-K1 Knockout cell line (MC38) Order

H2-K1 Knockout cell line (MC38)
Catalog#
YKO-M239
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
H2-K1 Knockout cell line (MC38)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
H2-K1 Knockout cell line（MC38）
Cell Morphology
Epithelial-like, Mixed: adherent and suspensio
Passage ratio
1：2-1：4
Species
Mouse
Gene Name
H2-K1
Gene ID
14972
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
Target region
Knockout region: Exon 1,Exon 2,Exon 3 (based on transcript H2-K1-201)
Validation: PCR+Sanger Sequencing
H2-K1 Knockout cell line (MC38) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MC38		 STR Info (Cell bank)
Cell Line:MC-38[EXPASY kerafast]
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 15.016.015.016.0
4-2 20.320.3
6-7 15.016.015.016.0
19-2 13.013.0
1-2 19.018.019.020.0
7-1 26.226.2
8-1 16.016.0
1-1 16.017.015.016.017.0
3-2 13.014.015.014.015.0
2-1 16.016.0
15-3 22.323.322.323.3
6-4 18.018.0
13-1 17.017.0
11-2 16.016.0
17-2 15.016.015.016.0
12-1 17.017.0
5-5 16.017.016.017.0
X-1 27.027.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the H2-K1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MC38 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, H2-K1 gene knockout H2-K1 Knockout cell line（MC38） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
H2-K1 Knockout cell line (MC38)
H2-K1 Knockout cell line (MC38)
Catalog#
YKO-M239
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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